05. Study on the possibility of eliminating phenol from pre-hydroyzate of lignocellulose in producing ethanol
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Abstract
This study focuses on the technique of eliminating phenol from pre-hydrolyzate to meet the requirement of fermentation to produce ethanol from Saccharomyces cerevisae 4182 yeast with the concentration of 106 CFU/ml at laboratory scale. The results showed that before pre-hydrolyzate was used for fermentation, phenol had been removed at 37oC. In this condition, laccase enzyme acted on the phenol fractions of lignin to cut Cα-Cβ bonds and aryl-aryl bonds resulting in removing of 46.61% phenol. The longer the hydrolysis, the higher the phenol removal efficiency (up to 59%). The phenol removal efficiency, however, reached the highest in the first 60 minutes. The lacccase enzyme was used to remove phenol at 20 ÷ 120 IU/g bagasse. The results indicated that at 70 IU/g bagasse, the phenol removal efficiency was at the highest efficiency 49.91% ± 2.5% and the ethanol extraction efficiency was at 76.27 ± 2%. The study results comfirmed the laccase's role in lignocellulose hydrolyzate extraction for ethanol production.
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References
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